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https://dora.health.qld.gov.au/qldresearchjspui/handle/1/4372| Title: | Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis | Authors: | Beissbarth, J. Anne Chang Marsh, R. L. Binks, M. J. Smith-Vaughan, H. C. Leach, A. J. |
Issue Date: | 2018 | Source: | 11, (1), 2018, p. 323 | Pages: | 323 | Journal: | BMC research notes | Abstract: | OBJECTIVE: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. RESULTS: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.L6243597702018-10-19 | DOI: | 10.1186/s13104-018-3429-z | Resources: | https://www.embase.com/search/results?subaction=viewrecord&id=L624359770&from=exporthttp://dx.doi.org/10.1186/s13104-018-3429-z | | Keywords: | infant;isolation and purification;male;microbiology;polymerase chain reaction;practice guideline;preschool child;female;retrospective study;standards;Australiachild;respiratory tract infection;Haemophilus infection;Haemophilus influenzae;human | Type: | Article |
| Appears in Sites: | Children's Health Queensland Publications |
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