Please use this identifier to cite or link to this item: https://dora.health.qld.gov.au/qldresearchjspui/handle/1/4347
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dc.contributor.authorCoin, L. J. M.en
dc.contributor.authorGraham, R.en
dc.contributor.authorHajkowicz, K.en
dc.contributor.authorBialasiewicz, S.en
dc.contributor.authorDuarte, T. P. S.en
dc.contributor.authorNguyen, S. H.en
dc.contributor.authorSukumaran, V.en
dc.contributor.authorStewart, A.en
dc.contributor.authorAppleton, S.en
dc.contributor.authorPitt, M. E.en
dc.contributor.authorBainomugisa, A.en
dc.contributor.authorJennison, A. V.en
dc.date.accessioned2022-11-07T23:51:51Z-
dc.date.available2022-11-07T23:51:51Z-
dc.date.issued2019en
dc.identifier.citation19, (1), 2019, p. 660en
dc.identifier.otherRISen
dc.identifier.urihttp://dora.health.qld.gov.au/qldresearchjspui/handle/1/4347-
dc.description.abstractBACKGROUND: Rapid diagnosis and appropriate treatment is imperative in bacterial sepsis due increasing risk of mortality with every hour without appropriate antibiotic therapy. Atypical infections with fastidious organisms may take more than 4 days to diagnose leading to calls for improved methods for rapidly diagnosing sepsis. Capnocytophaga canimorsus is a slow-growing, fastidious gram-negative bacillus which is a common commensal within the mouths of dogs, but rarely cause infections in humans. C. canimorsus sepsis risk factors include immunosuppression, alcoholism and elderly age. Here we report on the application of emerging nanopore sequencing methods to rapidly diagnose an atypical case of C. canimorsus septic shock. CASE PRESENTATION: A 62 year-old female patient was admitted to an intensive care unit with septic shock and multi-organ failure six days after a reported dog bite. Blood cultures were unable to detect a pathogen after 3 days despite observed intracellular bacilli on blood smears. Real-time nanopore sequencing was subsequently employed on whole blood to detect Capnocytophaga canimorsus in 19 h. The patient was not immunocompromised and did not have any other known risk factors. Whole-genome sequencing of clinical sample and of the offending dog's oral swabs showed near-identical C. canimorsus genomes. The patient responded to antibiotic treatment and was discharged from hospital 31 days after admission. CONCLUSIONS: Use of real-time nanopore sequencing reduced the time-to-diagnosis of Capnocytophaga canimorsus in this case from 6.25 days to 19 h. Capnocytophaga canimorsus should be considered in cases of suspected sepsis involving cat or dog contact, irrespective of the patient's known risk factors.L6288979452019-08-19 <br />2020-07-28 <br />en
dc.language.isoenen
dc.relation.ispartofBMC infectious diseasesen
dc.titleRapid diagnosis of Capnocytophaga canimorsus septic shock in an immunocompetent individual using real-time Nanopore sequencing: a case reporten
dc.typeArticleen
dc.identifier.doi10.1186/s12879-019-4173-2en
dc.subject.keywordsisolation and purificationen
dc.subject.keywordsantiinfective agentanimalen
dc.subject.keywordsbites and stingsen
dc.subject.keywordsCapnocytophagaen
dc.subject.keywordscase reporten
dc.subject.keywordscaten
dc.subject.keywordscomplicationen
dc.subject.keywordsDNA sequenceen
dc.subject.keywordsdogen
dc.subject.keywordsdrug effecten
dc.subject.keywordsfemaleen
dc.subject.keywordsgeneticsen
dc.subject.keywordsGram negative infectionen
dc.subject.keywordshumanen
dc.subject.keywordsimmunocompromised patienten
dc.subject.keywordsimmunologyen
dc.subject.keywordsmicrobiologyen
dc.subject.keywordsmiddle ageden
dc.subject.keywordsnanoporeen
dc.subject.keywordsseptic shocken
dc.relation.urlhttps://www.embase.com/search/results?subaction=viewrecord&id=L628897945&from=exporthttp://dx.doi.org/10.1186/s12879-019-4173-2 |en
dc.identifier.risid2288en
dc.description.pages660en
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeArticle-
Appears in Sites:Children's Health Queensland Publications
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