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  2. Hospital and Health Services
  3. Children's Health Queensland
  4. Children's Health Queensland Publications
Please use this identifier to cite or link to this item: https://dora.health.qld.gov.au/qldresearchjspui/handle/1/4346
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dc.contributor.authorMacdonald, J.en
dc.contributor.authorHeney, C.en
dc.contributor.authorPaterson, D. L.en
dc.contributor.authorTrembizki, E.en
dc.contributor.authorWang, C. Y. T.en
dc.contributor.authorWhiley, D. M.en
dc.contributor.authorZowawi, H. M.en
dc.contributor.authorIrwin, Adamen
dc.contributor.authorHarris, P. N. A.en
dc.contributor.authorAyfan, A. K. S.en
dc.date.accessioned2022-11-07T23:51:51Z-
dc.date.available2022-11-07T23:51:51Z-
dc.date.issued2021en
dc.identifier.citation40, (11), 2021, p. 2447-2453en
dc.identifier.otherRISen
dc.identifier.urihttp://dora.health.qld.gov.au/qldresearchjspui/handle/1/4346-
dc.description.abstractCarbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid detection tools are essential to aid in patient management. We developed rapid and simple molecular tests to detect blaNDM-type and blaVIM-type carbapenemase genes using recombinase polymerase amplification (RPA) combined with a lateral flow detection. The tests could provide results in approximately 15 min when using DNA extracts, with limits of detection of 9.2 copies/μl for the blaNDM-type assay and 7.5 copies/μl for blaVIM-type assay, and successfully detected all isolates harbouring the carbapenemase encoding genes in a panel of 57 isolates. These RPA tests may be suitable for use in low-resource settings to tailor rapid implementation of infection control precautions and antibiotic stewardship.L20114750522021-05-24 <br />2022-02-03 <br />en
dc.language.isoenen
dc.relation.ispartofEuropean Journal of Clinical Microbiology and Infectious Diseasesen
dc.titleRapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow–based detectionen
dc.typeArticleen
dc.identifier.doi10.1007/s10096-021-04267-6en
dc.subject.keywordsPseudomonas aeruginosaen
dc.subject.keywordsrecombinase polymerase amplificationen
dc.subject.keywordssensitivity and specificityen
dc.subject.keywordsThermal Cycleren
dc.subject.keywordsnonhumanen
dc.subject.keywordscamerahigh performance liquid chromatographen
dc.subject.keywordsPCR assay kiten
dc.subject.keywordspolymerase chain reaction systemen
dc.subject.keywordscarbapenemaseen
dc.subject.keywordsampliconen
dc.subject.keywordsantimicrobial stewardshipen
dc.subject.keywordsarticleen
dc.subject.keywordsCitrobacter freundiien
dc.subject.keywordscontrolled studyen
dc.subject.keywordsDNA sequencingen
dc.subject.keywordsDNA stranden
dc.subject.keywordsDNA templateen
dc.subject.keywordsEnterobacter aerogenesen
dc.subject.keywordsEscherichia colien
dc.subject.keywordsflow cytometryen
dc.subject.keywordshigh performance liquid chromatographyen
dc.subject.keywordsinfection controlen
dc.subject.keywordsKlebsiellaen
dc.subject.keywordsKlebsiella oxytocaen
dc.subject.keywordsKlebsiella pneumoniaeen
dc.subject.keywordslimit of detectionen
dc.subject.keywordsmultiplex real time polymerase chain reactionen
dc.relation.urlhttps://www.embase.com/search/results?subaction=viewrecord&id=L2011475052&from=exporthttp://dx.doi.org/10.1007/s10096-021-04267-6 |en
dc.identifier.risid2543en
dc.description.pages2447-2453en
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeArticle-
Appears in Sites:Children's Health Queensland Publications
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