Differential expression of genes and receptors in monocytes from patients with cystic fibrosis

Abstract

Introduction: We previously reported defective alternative polarization (M2)of macrophages and early expression of classically polarized (M1)macrophage markers in unpolarized monocyte-derived macrophages (MDMs)in patients with cystic fibrosis (CF). The present study assessed whether the mechanism(s)underlying defective macrophage polarization resided in circulating monocytes. Methods: Monocyte subsets (classical, intermediate and non-classical), markers for monocyte activation (CD163)and recruitment (CD195), receptors/genes associated with macrophage differentiation and polarization were analyzed in CF and compared with healthy individuals. Results: No differences were observed in the monocyte subsets or in the expression of CD163 or CD195. Expression of the M-CSF receptor, TLR4, γC, IL-4Rα, IL-13Rα1, TIMP-1 and Cox-2 were higher in CF monocytes, albeit at low levels, whereas, LRP1, MMP9, MMP28 were downregulated compared to mooncytes from healthy individuals. Conclusions: Our data suggest that differences in CF monocytes may contribute to the reported CFTR-dependent defect in macrophage differentiation, polarization and function.L20010718092018-09-11 <br />2019-06-07 <br />