Please use this identifier to cite or link to this item: https://dora.health.qld.gov.au/qldresearchjspui/handle/1/1641
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dc.contributor.authorMacesic, Nenaden_US
dc.contributor.authorBlakeway, Luke Ven_US
dc.contributor.authorStewart, James Den_US
dc.contributor.authorHawkey, Janeen_US
dc.contributor.authorWyres, Kelly Len_US
dc.contributor.authorJudd, Louise Men_US
dc.contributor.authorWick, Ryan Ren_US
dc.contributor.authorJenney, Adam Wen_US
dc.contributor.authorHolt, Kathryn Een_US
dc.contributor.authorPeleg, Anton Yen_US
dc.date.accessioned2022-02-14T02:08:58Z-
dc.date.available2022-02-14T02:08:58Z-
dc.date.issued2021-
dc.identifier.citationMacesic N, Blakeway LV, Stewart JD, Hawkey J, Wyres KL, Judd LM, Wick RR, Jenney AW, Holt KE, Peleg AY. Silent spread of mobile colistin resistance gene mcr-9.1 on IncHI2 'superplasmids' in clinical carbapenem-resistant Enterobacterales. Clin Microbiol Infect. 2021 Dec;27(12):1856.e7-1856.e13. doi: 10.1016/j.cmi.2021.04.020. Epub 2021 Apr 26. PMID: 33915285.en_US
dc.identifier.urihttp://dora.health.qld.gov.au/qldresearchjspui/handle/1/1641-
dc.descriptionCairns & Hinterland Hospital and Health Service (CHHHS) affiliated author: James D. Stewart.en_US
dc.description.abstractmcr-9.1 is a newly described mobile colistin resistance gene. We have noted its presence in multiple species of carbapenem-resistant Enterobacterales (CRE) from our institution. We aimed to determine the clinical features, genomic context and phenotypic impact of mcr-9.1 carriage in a series of patients between 2010 and 2019. We identified 32 patients with mcr-9.1-carrying CRE isolates (mCRE) and collected demographic, antimicrobial exposure and infection data. Whole-genome sequencing (including short and long reads) was performed on 32 isolates. We assessed sequence similarity of mcr-9.1-harbouring plasmids, then compared our findings with plasmids for which sequence data were publicly available. There was no colistin exposure in patients prior to isolation of mCRE. mcr-9.1 was identified on IncHI2 plasmids across four different bacterial species and was co-located with blaIMP-4 in 23/30 plasmids studied. mCRE isolates did not demonstrate phenotypic colistin resistance, either at baseline or following sublethal colistin exposure, thus showing that mcr-9.1 alone is not sufficient for resistance. Publicly available sequence data indicated the presence of carbapenemase genes in 236/619 mcr-9.1-carrying genomes (38%). IncHI2 plasmids carrying mcr-9.1 and carbapenemase genes were detected in genomes from North America, Europe, North Africa, Asia and Oceania. Spread of mcr-9.1 in CRE from our institution was driven by IncHI2 'superplasmids', so termed because of their large size and their prolific carriage of resistance determinants. These were also detected in global CRE genomes. Phenotypic colistin resistance was not detected in our isolates but remains to be determined from global mCRE.en_US
dc.language.isoenen_US
dc.relation.ispartofClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseasesen_US
dc.subjectCarbapenemaseen_US
dc.subjectEnterobacter hormaecheien_US
dc.subjectKlebsiella oxytocaen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectLong-read sequencingen_US
dc.titleSilent spread of mobile colistin resistance gene mcr-9.1 on IncHI2 'superplasmids' in clinical carbapenem-resistant Enterobacteralesen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.cmi.2021.04.020-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
Appears in Sites:Cairns & Hinterland HHS Publications
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