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DC Field | Value | Language |
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dc.contributor.author | Batoon, Lena | en |
dc.contributor.author | Millard, Susan Marie | en |
dc.contributor.author | Wullschleger, Martin Eduard | en |
dc.contributor.author | Preda, Corina | en |
dc.contributor.author | Wu, Andy Chiu-Ku | en |
dc.contributor.author | Kaur, Simranpreet | en |
dc.contributor.author | Tseng, Hsu-Wen | en |
dc.contributor.author | Hume, David Arthur | en |
dc.contributor.author | Levesque, Jean-Pierre | en |
dc.contributor.author | Raggatt, Liza Jane | en |
dc.contributor.author | Pettit, Allison Robyn | en |
dc.date.accessioned | 2019-05-30T01:36:08Z | - |
dc.date.available | 2019-05-30T01:36:08Z | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | Biomaterials. 2019 Mar;196:51-66. doi: 10.1016/j.biomaterials.2017.10.033. Epub 2017 Oct 22. | en |
dc.identifier.uri | http://dora.health.qld.gov.au/qldresearchjspui/handle/1/932 | - |
dc.description.abstract | Osteal macrophages (osteomacs) contribute to bone homeostasis and regeneration. To further distinguish their functions from osteoclasts, which share many markers and growth factor requirements, we developed a rapid, enzyme-free osteomac enrichment protocol that permitted characterization of minimally manipulated osteomacs by flow cytometry. Osteomacs differ from osteoclasts in expression of Siglec1 (CD169). This distinction was confirmed using the CD169-diphtheria toxin (DT) receptor (DTR) knock-in model. DT treatment of naïve CD169-DTR mice resulted in selective and striking loss of osteomacs, whilst osteoclasts and trabecular bone area were unaffected. Consistent with a previously-reported trophic interaction, osteomac loss was accompanied by a concomitant and proportionately striking reduction in osteoblasts. The impact of CD169+ macrophage depletion was assessed in two models of bone injury that heal via either intramembranous (tibial injury) or endochondral (internally-plated femoral fracture model) ossification. In both models, CD169+ macrophage, including osteomac depletion compromised bone repair. Importantly, DT treatment in CD169-DTR mice did not affect osteoclast frequency in either model. In the femoral fracture model, the magnitude of callus formation correlated with the number of F4/80+ macrophages that persisted within the callus. Overall these observations provide compelling support that CD169+ osteomacs, independent of osteoclasts, provide vital pro-anabolic support to osteoblasts during both bone homeostasis and repair. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.ispartof | Biomaterials | en |
dc.subject | bone structure | en |
dc.subject | fracture treatment | en |
dc.subject | macrophage | en |
dc.subject | Osteoblast | en |
dc.title | CD169+ macrophages are critical for osteoblast maintenance and promote intramembranous and endochondral ossification during bone repair | en |
dc.type | Article | en |
dc.identifier.doi | 10.1016/j.biomaterials.2017.10.033 | - |
item.cerifentitytype | Publications | - |
item.openairetype | Article | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Sites: | Metro North HHS Publications |
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