Please use this identifier to cite or link to this item: https://dora.health.qld.gov.au/qldresearchjspui/handle/1/8893
Title: Development and validation of an HPTLC–DPPH assay and its application to the analysis of honey
Authors: Md Khairul Islam
Tomislav Sostaric
Lee Yong Lim
Katherine Hammer
Cornelia Locher
Issue Date: 1-Jun-2020
Journal: Journal of Planar Chromatography - Modern TLC
Abstract: Using honey as a model sample, the aim of this study is to develop and validate a simple, rapid screening tool that allows for the visualization of constituents that contribute to the antioxidant activity of a complex natural product and to quantify their individual antioxidant effects even if their chemical identity is unknown. The method employs a validated analysis, which is based on the separation of constituents using high-performance thin-layer chromatography (HPTLC) followed by their visualization using DPPH* (2,2-diphenyl-1-picrylhydrazyl) as derivatizing reagent and the quantification of the antioxidant activity of individual bands expressed as gallic acid equivalents.
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Queensland Health Publications

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