Geographic consistency in dominant, non-typeable Haemophilus influenzae genotypes colonising four distinct Australian paediatric groups: a cohort study

Abstract

Non-typeable Haemophilus influenzae (NTHi)-associated ear and respiratory diseases (including pneumonia) represent a major health burden in many parts of the world. NTHi strains retrieved from the upper airways commonly reflect those found in the lower airways. Despite growing genomic and genotyping data on NTHi, there remains a limited understanding of global and regional NTHi population structures. The aim of this study was to determine whether nasopharyngeal carriage in four Australian paediatric groups at varying risk of NTHi colonisation was dominated by the same NTHi genotypes. Genotyping data generated by PCR-ribotyping were evaluated for 3070 NTHi isolates colonising the nasopharynges of Aboriginal and non-Aboriginal children enrolled in four longitudinal studies in three separate urban and remote regions of Australia. Several NTHi PCR-ribotypes dominated in nasopharyngeal carriage, irrespective of study setting. Principal coordinates analysis confirmed a cluster of common PCR-ribotypes among all cohorts. In conclusion, we identified dominant PCR-ribotypes common to geographically disparate Australian paediatric populations. Future genomic analyses will shed further light on the precise factors underlying the dominance of certain NTHi strains in nasopharyngeal carriage.eCollection. Cited Medium: Print. NLM ISO Abbr: Pneumonia (Nathan). PubMed Central ID: PMC5471799. Linked References: J Clin Microbiol. 2014 May;52(5):1352-7. (PMID: 24501028); J Pediatr. 2010 Dec;157(6):1001-5. (PMID: 20656297); Respir Med. 2007 Aug;101(8):1633-8. (PMID: 17467966); PLoS One. 2012;7(3):e34083. (PMID: 22470516); BMC Genomics. 2015 Aug 27;16:641. (PMID: 26311542); FEMS Immunol Med Microbiol. 2001 Aug;31(2):137-43. (PMID: 11549421); Lancet Infect Dis. 2014 Dec;14(12):1281-92. (PMID: 25012226); BMC Ear Nose Throat Disord. 2013 Oct 08;13(1):12. (PMID: 24099576); Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5439-44. (PMID: 24706866); Pediatr Infect Dis J. 2006 Sep;25(9):782-90. (PMID: 16940834); Med J Aust. 2010 Jan 4;192(1):24-9. (PMID: 20047544); Infect Genet Evol. 2013 Mar;14:125-36. (PMID: 23266487); J Clin Microbiol. 1995 May;33(5):1192-5. (PMID: 7542264). Linking ISSN: 22006133. Subset: PubMed not MEDLINE; Date of Electronic Publication: 2016 Aug 18. Current Imprints: Publication: 2016->: London : BioMed Central; Original Imprints: Publication: [Nathan], Qld. : Griffith University ePress, [2012]- <br />