The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus

Abstract

The eukaryotic translation factor eEF1A assists replication of many RNA viruses by various mechanisms. Here we show that down-regulation of eEF1A restricts the expression of viral genomic RNA and the release of infectious virus, demonstrating a biological requirement for eEF1A in the respiratory syncytial virus (RSV) life cycle. The key proteins in the replicase/transcriptase complex of RSV; the nucleocapsid (N) protein, phosphoprotein (P) and matrix (M) protein, all associate with eEF1A in RSV infected cells, although N is the strongest binding partner. Using individually expressed proteins, N, but not P or M bound to eEF1A. This study demonstrates a novel interaction between eEF1A and the RSV replication complex, through binding to N protein, to facilitate genomic RNA synthesis and virus production.eCollection. Cited Medium: Internet. NLM ISO Abbr: PLoS One. PubMed Central ID: PMC4257679. Linked References: J Gen Virol. 2001 Dec;82(Pt 12):2935-43. (PMID: 11714969); Elife. 2014;3:e03164. (PMID: 25233275); J Infect. 2002 Jul;45(1):10-7. (PMID: 12217725); J Biol Chem. 2003 Jul 4;278(27):24388-98. (PMID: 12700232); Virology. 2004 Mar 30;321(1):36-46. (PMID: 15033563); Virology. 2004 Mar 30;321(1):47-56. (PMID: 15033564); J Virol. 2004 Apr;78(8):4363-9. (PMID: 15047850); Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5952-7. (PMID: 15069200); Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7648-52. (PMID: 8052636); J Virol. 1997 Sep;71(9):6433-44. (PMID: 9261361); Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1449-54. (PMID: 9465035); Virus Res. 1998 Jan;53(1):13-25. (PMID: 9617766); J Biol Chem. 1999 Jan 8;274(2):666-72. (PMID: 9873000); J Virol. 1999 Jan;73(1):251-9. (PMID: 9847328); N Engl J Med. 2005 Apr 28;352(17):1749-59. (PMID: 15858184); Nature. 2006 Mar 23;440(7083):556-60. (PMID: 16554823); Virology. 2006 Mar 30;347(1):100-8. (PMID: 16386773); FEMS Microbiol Rev. 2006 Sep;30(5):692-705. (PMID: 16911040); J Virol. 2006 Sep;80(17):8459-68. (PMID: 16912296); J Biomed Sci. 2006 Nov;13(6):861-74. (PMID: 16927014); Nat Rev Mol Cell Biol. 2007 Oct;8(10):761-73. (PMID: 17786152); Mol Biol Cell. 2008 Dec;19(12):5296-308. (PMID: 18799616); Virology. 2009 Mar 1;385(1):245-60. (PMID: 19131084); Virology. 2009 Jul 20;390(1):71-8. (PMID: 19464723); Arch Virol. 2010 Feb;155(2):263-8. (PMID: 20012112); Lancet. 2010 May 1;375(9725):1545-55. (PMID: 20399493); J Biol Chem. 2010 Jul 9;285(28):21209-13. (PMID: 20444696); PLoS Pathog. 2010;6(11):e1001175. (PMID: 21079685); Virus Res. 2011 Dec;162(1-2):80-99. (PMID: 21963675); PLoS Pathog. 2011 Dec;7(12):e1002438. (PMID: 22194687); Pediatrics. 2012 Jan;129(1):55-61. (PMID: 22184649); Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9587-92. (PMID: 22628567); Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834); J Gen Virol. 2002 Apr;83(Pt 4):753-7. (PMID: 11907323). Linking ISSN: 19326203. Subset: MEDLINE; Date of Electronic Publication: 2014 Dec 05. ; Original Imprints: Publication: San Francisco, CA : Public Library of Science <br />