Probe‐capture targeted next‐generation sequencing: A novel approach for pathogen and antimicrobial resistance detection in sepsis

Abstract

The article focuses on the advancements in diagnostic methods for bloodstream infections (BSIs) and sepsis, highlighting the limitations of traditional blood culture (BC) methods and the potential of targeted next-generation sequencing (tNGS) using probe-capture techniques. Sepsis, a life-threatening condition caused by an immune response to infection, resulted in 11 million fatalities globally in 2017. While conventional BC methods have a low detection rate and long turnaround times, tNGS offers faster and more sensitive pathogen identification, significantly improving clinical outcomes. Despite its advantages, the article notes challenges in broader clinical implementation, including the need for known reference genomes and potential bioinformatics complications. Overall, tNGS, particularly probe-capture, is positioned as a promising tool for enhancing diagnostics and antimicrobial resistance (AMR) stewardship in critical care settings.