Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

Abstract

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:•Separation of trehalulose fraction•Lyophilization•Confirmation of trehalulose The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.